Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan
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چکیده
Chitosan was depolymerized either by HCl hydrolysis or enzymatic degradation with a commercial preparation Pectinex Ultra Spl. The chitooligosaccharides released by both methods were selectively precipitated in methanol solutions and characterized using MALDI-TOF mass spectrometry. Differences between the two methods were detected and concerned the degrees of polymerization of the fragments produced and their acetylation. The enzymatic method yielded shorter fragments with a higher proportion of fully deacetylated chitooligomers. Conversely, a t ©
منابع مشابه
مقایسه ی خواص ضد باکتری کیتین، کیتوزان و کیتوالیگومرهای به دست آمده از پوسته ی میگوی سفید سرتیز (Metapenaeus affinis )
Background and purpose: Chitin is one of the main components of crustaceans’ exoskeleton. Chitosan is produced by deacetylation of chitin. Molecular weight and degree of deacetylation play important roles in biological activity of chitin, chitosan, and their derivatives. So far, various derivatives of chitin and chitosan are obtained. The purpose of this study was to prepare derivatives o...
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